Leica DFC9000 Digital Microscope Camera

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Get closer to reality


sCMOS camera Leica DFC9000

Imagine you could image live cells as close as possible to their native state. Capture even faint fluorescent signal you currently miss. And discover what happens in the time between two subsequent images. Imagine you could get a better view of what really happens in cells!

The Leica DFC9000 sCMOS camera will turn your imagination into reality. This monochrome microscope camera with newest scientific CMOS (sCMOS) sensor technology for fluorescence imaging enables you to image your cells under near-native conditions.

  • Protect your cells with short exposure times
  • Capture highly dynamic processes with high-speed acquisition
  • Get more information in one image with large field of view

This Introduction to Camera Technology helps you judge camera performance.


Every photon counts

The Leica DFC9000 is built with a third-generation sCMOS sensor, currently state-of-the-art on the market. It performs with a quantum efficiency* of up to 82%.

  • Capture even the faintest, previously undetected fluorescence signals
  • See processes in the cell in the most realistic detail without unhealthy overexpression of the GFP-coupled protein

Reduce exposure times to a minimum

  • Protect your cells, reducing stress, and minimizing phototoxicity effects
  • Generate more reliable results with happy GFP-expressing cells

Protect your cells with high near-infrared sensitivity

  • Use red-emitting fluorescence tags to further reduce cell damage
  • Observe your cells with an outstanding spectral response of more than 60% quantum efficiency at 750 nm wavelength

*quantum efficiency depends on wavelength of light

Paramecium expressing GFP-Epiplasmin. Polyglycylated Tubulin immunolabeled with Cy5. Nucleus stained with Hoechst 33258 (blue). Acquisition with LAS X. Specimen courtesy of Dr. A. Aubusson-Fleury; CGM-CNRS, FranceCatcher of the light

The Leica DFC9000 is designed for high-end fluorescence imaging of live cells such as time-lapse experiments.

Less noise means a better image. In technical terms, a high signal-to-noise ratio (SNR) provides you with a crisp fluorescence signal against a dark background.

The Leica DFC9000 is a highly sensitive sCMOS camera:

  • High quantum efficiency over the entire light spectrum with a maximum of 82%
  • Low read noise of 0.9 e- at 216 MHz pixel clocking rate
  • Cooled sensor; cooling to 0° Celsius by air circulation

These features make the Leica DFC9000 also ideally suited for our lightsheet system Leica TCS SP8 DLS.

Vero cells marked with DAPI, Anti-Tubulin, and Anti-Pmp-70 (peroxisomesStandard imaging port matches 19 mm diagonal of DFC9000 sensor. See 41% more at a glance and be 30% faster during tile scans!

Matchmaking by Leica

See more with imaging systems from one source: Leica Microsystems provides you with imaging systems specifically designed to take full advantage of sCMOS sensors to ensure that you do not miss any data.

The uniquely perfect match

  • 19 mm diagonal of the Leica DFC9000’s sCMOS sensor
  • 19 mm camera port with full optical correction in many Leica research microscopes

Acquire fewer images

  • If you want to acquire tile scans or mosaics, fewer individual images are required
  • Save time and protect your samples

    Conduct your imaging orchestra

    The Leica DFC9000 is part of a carefully tuned microscope platform capable of being triggered at high speed to protect cells from unnecessary light and to have filter changes take place in shortest time.

    Leica Application Suite X software

    Triggering is based on Leica Microsystems’ flexible electronic box system and Leica Application Suite X (LAS X) software.

    • Flexible: Trigger up to 2 cameras with the electronic box
    • Simultaneous: Combine the camera with DualView or Gemini for simultaneous acquisition of 2 wavelengths
    Leica DMi8 Inverted Microscope with Leica DFC9000 Microscope Camera